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| 실험적 IgA 신병증에서의 사구체 기저막 음이온 부위의 전자현미경적 미세변화 |
| 박성뱁, 김준식 , 박관규 |
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| Abstract |
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We studied the ultrastructual alteration of glomerular anionic sites in experimental model of IgA nephropathy, using polyethyleneimine (PEI) as a cationic probe. IgA nephropathy was induced in a group of ICR mice by oral administration of bovine gamma globulin and polio vaccine. The presence of anionic sites in the lamina rara interna and lamina densa, as well as in the lamina rara externa, of the normal glomerular basement membrane. The anionic sites in the lamina rara interna and lamina densa of normal glomerular basement membrane were always less numerous and less regulary distributed than anionic sites in the lamina rara extema. Ultrastructally, electron dense deposits were noted in mesangium, par- amesangium, subendothelium and intramembranous region. Prominent common findings in the glomeruli were few PEI particles in the mesangial and subendoth- elial areas where were densely electron deposited and marked reduction in glomerular anionic sites in the regions with deposits. Mesangial deposits did not alter the anionic sites of glomerular basement membrane, however some deposits had a faint and incomplete layer of anionic site around them. Subendothelial electron dense deposits were associated with defects in the anionic sites of the lamina rara interna, but the suben- dothelial deposits had little influence on the anionic sites in neighboring lamina rara externa. These results suggest that structural alteration of glomerular anionic sites in experimental IgA nephropathy was associated with immune deposition of the glomerular basement membrane and mesangial area. |
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