Korean Journal of Nephrology 1996;15(2):117-122.

사람 근위 세뇨관 세포에서 HDL에 의한 Endothelin - 1 분비 증가에 대한 Protein Tyrosine Kinase의 역할

송주홍

Abstract

To evaluate whether high density lipoprotein (HDL) stirnulates endothelin-1 release by cultured human renal proximal tubular cells (Hptc) through protein tyrosine kinase dependent pathway, Hptc were cultured from human nephrectomy tissue and preincubated for 1 hour with 25 Mmi of genistein (GS) or for 18 hours with 1 Mm of herbimy cin (HB) in DMEM/F12 with or without 5% fetal calf serum (FCS). hPTC were also preincubated with 12.5, 25, 50 pM of GS or 0.5, 1, 2 pM of HB in 5% FCS- containing DMEM/F12 as the same way. 200 m g/ml of HDL was added into each well except for control. ET-1 in the supernatant was measured by radio- immunoaney after 24 hours of HDL exposure. In serum-free media, HDL increased ET-1 production significantly from 25.1±3.7 to 100.6 ± 5.3 pg/mg cell protein/24hr, but GS and HB did not suppress HDL stimulated ET-1 production. Compared with ET-1 production in serum free media, 5% FCS increased ET-@1 production significantly from 25.1± 3.7 to 180.5± 6.0 pg/mg cell protein/24hr. In 5% FCS-containing culture media, HDL increased ET-1 production significantly from 180.5±6.0 pg/mg cell protein/24hr to 261.7 ± 12.0 pg/mg cell protein/24hr. 25 m M of GS suppressed HDL induced ET-1 production signi- ficantly to 149.7±12.6 pg/mg cell protein/24hr and 1 m M of HB inhibited HDL-stimulated ET-1 pro- duction to 202.1±16.0 pg/mg cell protein/24hr. HDL induced ET-1 production was decreased gradually as the concentration of GS and HB were increased. These results demonstrate that activation of protein tyrosine kinase plays a role in the HDL-stirnulated ET-1 production by hPTC cultured in media containing 5%.