Korean Journal of Nephrology 2007;26(2):137-154.
Original Article : Differential Gene Expression According to the Size of Glomeruli in Experimental Diabetic Nephropathy
Seung Jae Kwak1, Dong Sub Jung1, Jin Ju Kim1, Jin Ji Li, M.D.1, Seung Hyeok Han, M.D.2,Tae Hee Lee, M.D.2, Dong Ki Kim, M.D.2 , Sung Jin Moon, M.D.2,Dae Suk Han, M.D.2 and Shin-Wook Kang, M.D.2
Department of Internal Medicine1 Yonsei University College of Medicine, Brain Korea 21 Department of Internal Medicine2
기초연구 : 실험적 당뇨 흰쥐에서 사구체의 크기에 따른 유전자 발현의 차이
곽승재1
연세대학교 의과대학 Brain Korea 211, 내과학교실2
Abstract
Purpose
Although a few gene-profiling studies with whole renal tissue have been described in experimental diabetic nephropathy, there is only one microarray study using diabetic glomeruli. Furthermore, hypertrophic glomeruli have not been explored. The purpose of this study is to elucidate gene expression profiles of hypertrophic glomeruli in early diabetic nephropathy.
Methods
Forty-male Sprague-Dawley rats were injected with diluent (N=20) or streptozotocin intraperitoneally (DM, N=20) and were sacrificed at 6- and 12-week. Glomeruli were isolated by sieving technique. Glomeruli from 125 and 75 m sieves were classified into large (hypertrophic, DM-LG) and small glomeruli (DM-SG), respectively. After RNA extraction, hybridization was performed on the Rat cDNA 5K chip in triplicate, and slides were analyzed. The significant genes were selected using significant analysis of microarray.
Results
At 6-week, hierarchical clustering revealed that gene expression profiles of DM-LG were different from those of DM-SG, whereas DM-SG and C glomeruli showed similar gene expression pattern. In contrast, gene expression profiles at 12-week were similar between DM-LG and DM-SG, whereas C glomeruli showed different gene expression pattern from DM glomeruli. At 6-week, a total of 207 genes showed greater than 1.5-fold differential expression. 149 genes were upregulated, whereas 58 were downregulated in DM-LG. On the other hand, differential gene expression greater than 1.4 - fold was observed in 37 genes at 12-week, upregulated in 26 and downregulated in 11.
Conclusion
These results suggest that the gene expression profiles of DM-LG are different from DM-SG, and the gene expression patterns change with the progression of diabetic nephropathy. Key Words : Diabetic nephropathy, Kidney glomerulus, Hypertrophy, Microarray analysis, Gene expression
Key Words: Diabetic nephropathy, Kidney glomerulus, Hypertrophy, Microarray analysis, Gene expression


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