| The Anti-apoptotic Effect of Adrenomedullin on Vascular Endothelial Cells in Radiocontrast Media-induced Apoptosis |
| Kyung Pyo Kang, M.D.1, 2, Ae Sin Lee, B.S.1, 2, Yu Jin Jung, B.S.1, 2, Duk Hoon Kim, M.S.1, 2 Kang Suk Ko, M.D.1, 2, Kyoung Hwa Choi, M.D.1, 2, Young Wook Jeong, M.D.1, 2, Sik Lee, M.D.1, 2 Sung Kwang Park, M.D.1, 2, Mi Jung Sung, Ph.D.3 and Won Kim, M.D. |
Department of Internal Medicine1
Research Institute of Clinical Medicine2 Chonbuk National University Medical School, Jeonju, Food function Research Division3
Korea Food Research Institute, Sungnam, Republic of Korea |
| 원저 : 혈관내피세포에서 방사선 조영제에 의해서 유발된 아포프토시스에 대한 adrenomedullin의 세포보호 효과 |
| 강경표1, 2•이애신1, 2•정유진1, 2•김덕훈1, 2•고강석1•최경화1 정영욱1•김이식1•이 식1, 2•박성광1, 2•성미정3•김 원1, 2 |
| 전북대학교 의학전문대학원 내과학교실1, 임상연구소2, 한국식품연구원 식품기능본부3 |
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| Abstract |
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Purpose : Because vascular endothelial cells play a pivotal role in the vascular diseases, damage of vascular endothelial cells lead to progression of vascular disease. Apoptotic damage of cells is an important mechanism in vascular disease. Therefore, several growth factors that have antiapoptotic effect may have a protective role in maintaining a cell function in apoptotic cell injury. In this study, we examined the effects of adrenomedullin on apoptosis in iopromide-induced endothelial cell injury.
Methods : Human umbilical vein endothelial cells were incubated with nonionic radiocontrast agent, iopromide and/or adrenomedullin. Apoptosis was assessed quantitatively using FACScan after annexin V-FITC and propidium iodide staining, and by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) stain. Signaling pathway was evaluated by Western blot analysis of phospho-Akt and Akt.
Results : Iopromide-induced apoptosis in human umbilical vein endothelial cells was increased in a dose-dependent manner. Adrenomedullin prevented iopromide-induced apoptosis in human umbilical vein endothelial cells in a dose dependent manner. Wortmannin, phosphatidylinositol 3-kinase inhibitor, decrease the adrenomedullin-induced antiapoptotic effect.
Conclusion : These results suggest that adrenomedullin protects vascular endothelial cells from iopromide-induced apoptosis by regulating the activity of Akt.
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| Key Words:
Contrast media, Iopromide, Apoptosis, Adrenomedullin |
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