Korean Journal of Nephrology 1996;15(1):1-24.

배양된 인형 Mesangial 세포에서 배양액 차이에 따른 Interleukin 6의 변화

진동규

Abstract

The use of intravenous immunoglobulin (IVIG) in various immunologic disorders has been reported to cause renal damage. However, the extent and mechanism of its nephrotoxicity has not been studied adequately. So that present experimental study has been undertaken to determine the extent and mechanisms of nephrotoxicity of IVIG given to rabbits. In the first experiment, rabbits weighting 1.5-2.0 kg were used for experimental animal and human IVIG (IV-Globulin, Green Cross, Korea) was given in a dose of 3 gm/kg through ear vein. Group I (n=5) reveived IVIG alone. Group II (n=5) received pretreatment with methylprednisolone (30 mg/kg) and Group III (n=5) pretreatment withverapamil(0.15 mg/kg) in addition to IVIG. Twelve hours after IVIG infusion, animals were sacrificed and kidneys were taken for histopathologic examination. BUN, determined before and 12 hours after IVIG infusion, showed significant elevation (p<0.05) and serum Na' significant decrease (p<0.05) in all three groups. PRA, determined 12 hours after IVIG infusion in Group I showed decreased value campared to the value of "before" IVIG infusion (12.9±5.7 vs 3.5±3.4 ng/ml/hr, p<0.05) and serum prostaglandin E" by RIA did not show any significant changes in the experimental groups campared to the control. Analysis of proteinuria by SDS-PAGE method after IVIG infusion showed mainly tubular pattem in all three groups. Histopathologic changes observed in experimental animals were mainly confined to the renal tubules, showing patchy areas of necrosis. In the second experiment, rabbits weighting 1.3 Kg were used for experimental animal and rabbit IVIG(Sigma, USA) was given in a dose of 2.5 gm/ kg through ear vein. Twelve hours after IVIG infusion, animals were sacrificed and kidneys were taken for histopathologic examination, which showed the same pathologic changed as those observed in the 1st experimental animals. In conclusion, present study indicates that IVIG can cause renal tubular damages in rabbits. And this nephrotoxic effect can not be preventable by pretreatment with methylprednisolone nor veraparnil.