Korean Journal of Nephrology 1998;17(4):545-552.

만성 요독증 흰쥐에서 수면장애와 시상하부의 c-fos Protein 발현 양상과의 관계 (The Relationship between Sleep c-fos Protein in Hypothalamus in Chronic Uremic Rats)

김영옥, 박철휘, 송호철, 윤선애, 진동찬, 김용수, 김석영, 최의진, 장윤식, 방병기 (Young Ok Kim, Chul Whee Park, Ho Chul Song, Sun Ae Yoon, Dong Chan Jin, Yong Soo Kim, Suk Young Kim, Euy Jin Choi, Yoon Sik Chang and Byung Kee Bang)

Abstract

The activation of c-fos protein(FOS) in ventrol- ateral preoptic neuron(VLPO) induces sleep(Science 271; 216-219, 1996). Although sleep disturbance are very common in patients with chronic renal failure, its mechahanism of sleep disturbance is not clear. The purpose of this study was to evaluate sleep dissturbance and expression of FOS in VLPO of chronic uremic rats. Chronic uremic rats were induced by 5/6 nephrectomized rnodel. After 4 weeks, blood urea nitrogen and serum creatinine in uremic rats(n=14 were higher than in control(n=17) (blood urea nitrogen; 73.6+-24.8mg/dl vs. 23.3+-2.9 mg/dl, P<0.001 serum creatinine', 1.49+-0.42mg/dl vs. 0.66+-0.12mg/dl, P<0.001). General activity was counted with infrared during day time(08:00-20:00) and night time(20:00-08:00). Rats were killed at 10:00 or 16:00 during day time(uremic rats 7, control 9) and at 22:00 during night time(uremic rats 7, control 7). The expression of FOS in VLPO was examined with immunohistochernical method. The number of day tirne general activity in uremic rats was significantly higher than in control (458+-185 vs. 222+-41, P<0.001), and the number of night time general activity in uremic rats was lower than in control(949+- 430 vs. 1618+-261, P<0.001). During day time, the number of FOS immunoreac- tive cell in uremic rats was lower than in control (18.4+-5.3 vs. 42.8+-6.3, P<0.001), but there was no difference between 2 groups at night time(10.8+-8.4 vs 12.5+-5.1, P=0.62). There was strong negative correlation between the number of activity and the number of FOS immunoreactive cell in control(r= -0.93, P<0.001), but there was no correlation in uremic rats. This study shows that sleep disturbance in chronic uremic rats might be related to decrease of expression of FOS in VLPO.